General Framework
Main National and International Collaborations and Reference Groups
Areas of Research
Main ongoing Research Activities and Projects
Methodologies and Facilities
Selected References
Internet Links in Cardiology

By definition, only the research areas where the specificity of available techniques could "make a difference" are covered in this section.
6.1. Experimental and Clinical Pharmacology
6.2. Clinical Studies
6.3. Medical Informatics and Statistics

  • 6.1. Experimental and Clinical Pharmacology

    a. Animal models

    * tracheal intubation and mechanical ventilation of mice and rats;
    * acute MI induced in rats and mice by left coronary artery ligation;
    * acute ischemia and reperfusion in the anesthetized rat;
    * renovascular hypertension induced by abdominal aorta constriction or by two kidneys one clip procedure;
    * conscious rats with venous or arterial cannulas implanted to draw serial blood samples for neurohormone and drug assays;
    * implanted minipumps for long-term drug administration in conscious rats.

    b. Methods

    * arterial blood pressure measurement with tail cuff non-invasive method in conscious rats;
    * surface ECG;
    * left ventricular pressures measured with microtip transducers in anesthetized rats and mice;
    * histo-morphometric analysis of rodent cardiac tissue sections (fibrosis, cardiomyocytes cross sectional area, ventricular morphology, infarction area);
    * high performance liquid chromatographic assays for the measurement in biological samples of catecholamines and cardiovascular drugs (spectrophotometric, fluorimetric and electrochemical detectors are available);
    * histologic staining methods (e.g. tetrazolium, Sirius red, hematoxilin-eosin, toluidine blue);
    * radioimmunoassays (RIA) for endothelin, plasma renin activity, angiotensin II brain natriuretic peptide (BNP) and aldosterone;

    c. Data analysis

    * computer-assisted methods to analyze hemodynamic data (digitizing tablet, VIEW II Gould);
    * standard computer softwares (i.e. EasyFit, MKMODEL, SAAM) for pharmacokinetic and pharmacodynamic analyses;
    * histo-morphometric analysis with ad-hoc written softwares for image analyzer (IBAS).

  • 6.2. Clinical Studies

    * Clinical networks implementation and management
    * Planning, management, monitoring of large scale trials according to GCP/ICH rules
    * Quality control of instrumental examinations: (12 lead electrocardiograms, two-dimensional echocardiograms, 24-h Holter monitoring, exercise stress testing and coronary angiograms)
    * ECG quantitative analysis with graphic digitizer
    * Quality of Life techniques and questionnaires

  • 6.3. Medical Informatics and Statistics

    a. Methodologies - Informatics
    * Data base design methodology
    * Data base programming
    * Automatic randomisation processes (by telephone or computerized network)
    * Telematics

    b. Methodologies - Statistics
    * Survival analysis
    * Analyses of variance, linear regression models, meta-analysis

    c. Software
    * Operating systems: UNIX (HP-UX, Linux), VMS, MSDOS, WINDOWS, MacOS
    * Programming languages: SQL, C, VISUAL BASIC, PHP
    * Programmable database management systems: INGRES, FOXPRO, ACCESS, EPI INFO
    * Telecommunication: INTERNET, WWW, Internet Information Server, Apache
    * Computer programmes for statistical analysis: SAS, SPSS, S-PLUS

    d. Hardware
    * HP 9000/800 D320
    * DEC Microvax 4000
    * PCs MSDOS Compatible
    * Macintosh
    * Netserver LH PRO

Home Page Department  Home Page Institute

Department of Cardiovascular Research
"Mario Negri" Institute for Pharmacological Research

Via Eritrea, 62 - 20157 Milano - Italy
Tel. +39-0239014482 Fax. +39-0233200049


Scientific Info:DepCardio
Technical Info:Enrico Nicolis